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Wondershare Dr.Fone Toolkit For Iso Pc Mac 10.8.9.86 FULL+Crack Download Windows.  .Oops!.[Determination of gallic acid in Umbelliferone and Calendulae extract from St. John's wort by HPLC].
A rapid method for determination of gallic acid in hydroalcoholic extract from St. John's wort by HPLC was developed, including the extraction of hydroalcoholic extract from St. John's wort, clean up by solid phase extraction on C18, determination of gallic acid by HPLC, and quantitative analyses were examined by an internal standard method. The results showed that the extraction rate of gallic acid in Umbelliferone and Calendulae (55 mg/g) was similar to that in naringenin (50 mg/g). The UPLC-MS/MS was used to determine, and the chromatographic analysis was carried out on a Hypersil ODS column (100 mm × 2.1 mm, 1.9 μm), aqueous solution and acetonitrile-water-acetic acid were used as the mobile phase. The total analysis time was 2 min, the analysis flow rate was 0.2 mL/min, the detection wavelength was 254 nm, and the column temperature was 30°C. The linear regression equation was Y=0.135X (r=0.999 6) in the range of 25-50 μg/mL. The repeatability of the determination for 25, 50, and 75 μg/mL were 0.17%, 0.25%, and 0.25%, respectively. The recoveries of the extraction of gallic acid was 92.5%-97.0%. The method is simple, rapid, and accurate, which is suitable for the determination of gallic acid in the various biological samples.Related
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Star Wars: The Clone Wars: Season Two PreviewPosted By Mike on March 24, 2008
Posted by Mike on March 24, 2008
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